DNA Purification
نویسنده
چکیده
منابع مشابه
Rapid purification of HU protein from Halobacillus karajensis
The histone-like protein HU is the most-abundant DNA-binding protein in bacteria. The HU protein non-specifically binds and bends DNA as a hetero- or homodimer, and can participate in DNA supercoiling and DNA condensation. It also takes part in DNA functions such as replication, recombination, and repair. HU does not recognize any specific sequences but shows a certain degree of specificity to ...
متن کاملRapid DNA Extraction Protocol from Stool, Suitable for Molecular Genetic Diagnosis of Colon Cancer
Colorectal cancer (CRC) is one of the most common forms of cancers in the world and is curable if diagnosed at the early stage. Analysis of DNA extracted from stool specimens is a recent advantage to cancer diagnostics. Many protocols have been recommended for DNA extraction from stool, and almost allof them are difficult and time consuming, dealing with high amount of toxic materials like phen...
متن کاملIsolation of genomic DNA from blood using a novel filter-based DNA purification technology.
A novel DNA purification technology is described that enables the purification of pure dsDNA from blood. When compared to existing DNA purification methods, the Whatman BioScience Purification System (WBPS) offers a fast and convenient way to recover high yields of DNA. WBPS is based on a unique filter system that entraps DNA within a matrix. This allows the process to be performed in a single ...
متن کاملIsolation and purification of sesquiterpene coumarins from Ferula assa-foetida and their in vitro interaction study with DNA
Background and objectives: Interaction of compounds with ct-DNA can affect the replication, protein synthesis and cell division. Different species of the genus Ferula (Apiaceae) have shown various biomedical applications for many centuries. Biological features of this genus such as cytotoxicity, antibacterial, antiviral, P-glycoprotein (P-gp) inhibitory and antiinflamm...
متن کاملEvaluation of methods for the extraction and purification of DNA of cultured Lactobacillus colony isolated from dairy products
Article Type Full Length Research Article Lactic acid bacteria are very significant to human health due to the production of some antimicrobial substances and ability to inhibit pathogenic bacteria. Furthermore, the bacteria are also used as starter culture in the production of various foods. The aim of this study was to evaluate five methods for the extraction and purification of DNA from 6 is...
متن کاملData of expression and purification of recombinant Taq DNA polymerase
Polymerase chain reaction (PCR) technique is widely used in many experimental conditions, and Taq DNA polymerase is critical in PCR process. In this article, the Taq DNA polymerase expression plasmid is reconstructed and the protein product is obtained by rapid purification, ("Rapid purification of high-activity Taq DNA polymerase" (Pluthero, 1993 [1]), "Single-step purification of a thermostab...
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تاریخ انتشار 2001